Hurler's syndrome: biosynthesis of acid mucopolysaccharides in tissue culture.
نویسندگان
چکیده
منابع مشابه
Mucopolysaccharides Produced in Tissue Culture
1. A method of mass tissue culture has been devised by which, in a relatively short period of time, samples large enough for chemical isolation of mucopolysaccharides can be obtained. 2. Chemical isolation of acid mucopolysaccharides from mass cultures of human fetal skin, human fetal bone, bovine fetal skin, and rat subcutaneous tissue has been carried out. It has been found that the fibroblas...
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A clonal strain of rat glial tumor cells was shown to produce the acid mucopolysaccharides, hyaluronic acid, chondroitin 4-SO(4), and heparan sulfate in tissue culture. The mucopolysaccharides were isolated both from the growth medium and the cells.
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changed, whereas hexosamine values were gradually increased and seemed to be correlated to total cell count, but not to the count of adhered cells on the glass wall. On the other hand, hexosamine and sialic acid contents of both the cells adhering to the glass wall were determined after washing with saline solution. The results are given in Table I. No remarkable difference in hexosamine and si...
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متن کاملAcid mucopolysaccharides in normal serum.
Mucopolysaccharide which contains uronic acid has recently been described in normal plasma (1, 2). A method for the quantitation of plasma acid mucopolysaccharide was described by Badin, Schubert and Vouras (1), based on the observation that chondroitin sulfate added to plasma is found in euglobulin precipitates (3). These authors reported an increased amount of acid mucopolysaccharide in the p...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1966
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.56.4.1310